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目的 研究解毒软肝汤对CCl4诱导的大鼠肝纤维化的预防与治疗作用 ,并探讨其作用机理。 方法 以CCl4诱导大鼠肝纤维化模型 ,造模前 2周及造模结束后分别给予相应药物灌胃 ,各持续 10周。实验结束后测定肝组织羟脯氨酸 (Hyp)、血清超氧化物歧化酶 (SOD)、丙二醛 (MDA)、肿瘤坏死因子α(TNF α)、转化生长因子 β1(TGF β1) ;光镜观察肝组织病理形态变化。 结果 解毒软肝汤预防和治疗组及秋水仙碱组Hyp、MDA、TNF α、TGF β1显著降低 (P <0 .0 1) ,SOD显著升高 (P <0 .0 1) ,胶原沉积明显减轻 (P <0 .0 1) ;且解毒软肝汤组疗效优于秋水仙碱组。 结论 解毒软肝汤具有确切的抗肝纤维化的作用 ,效果优于秋水仙碱 ,副作用较秋水仙碱小 ,对机体的损害较轻 ,具有较大的临床应用价值 ,其作用机理可能与抗脂质过氧化和抑制肝星状细胞 (HSC)活化有关。
Objective To study the preventive and therapeutic effects of Jiedu Ruangan Decoction on CCl4-induced hepatic fibrosis in rats, and to explore its mechanism of action. Methods Rat models of hepatic fibrosis were induced by CCl4. The rats were given intragastric administration for 2 weeks before the model establishment and after the completion of the model respectively, each lasting for 10 weeks. At the end of the experiment, hydroxyproline (Hyp), serum superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor α (TNFα) and transforming growth factor β1 (TGFβ1) were measured. Microscopic observation of pathological changes in liver tissue. Results Hyp, MDA, TNFα, TGFβ1 in Jieduengantang decoction group and colchicine group were significantly decreased (P < 0.01), SOD was significantly elevated (P <0.01), collagen deposition was obvious. Reduced (P <0. 0 1); and Jieduuangantang group was superior to colchicine group. Conclusion Jieduen Gangan Decoction has an anti-hepatic fibrosis effect, which is superior to that of colchicine. It has less side effects than colchicine and has less damage to the body. It has greater clinical application value, and its action mechanism may be related to resistance. Lipid peroxidation is associated with inhibition of hepatic stellate cell (HSC) activation.