综述了运用杂交授粉试验和分子生物学方法等技术确定梨品种自交不亲和基因型研究的技术进展,分析了这些技术在确定梨品种自交不亲和基因型方面的优点和不足之处,并初步探讨了研究前景。因为HV区氨基酸的不同,不同S基因型也有所差异。因此,除了在分子生物学的水平上进行研究外,其他方法如mRNA、蛋白质和杂交授粉等水平上的研究在确定S基因型上也同样重要。 The progress of research on identification of self-incompatibility genotypes of pear cultivars by using techniques such as cross-pollination and molecular biology was reviewed. The advantages and disadvantages of these techniques in identifying self-incompatible genotypes of pear cultivars , And initially explored the research prospects. Because of the different amino acids in the HV region, different S genotypes are also different. Therefore, in addition to research at the molecular biology level, studies on other approaches such as mRNA, protein and cross-pollination are equally important in determining the S genotype.