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作者用型特异性登革热病毒单克隆抗体间接免疫荧光技术,研究了持续感染1型登革热病毒的白纹伊蚊C6/36细胞,用3型登革热病毒再感染时的型间干扰情况。正常细胞用3型登革热病毒感染3天后,含3型登革热病毒抗原的细胞在90％以上。而1型登革热病毒持续感染的细胞,再用3型登革热病毒感染,结果只有0.1～1％的细胞含3型登革热病毒抗原。1型登革热病毒持续感染的细胞,在3型登革热病毒再感染前后用1～4型登革热病毒单克隆抗体染色,均有90％以上的细胞含荧光素。在1型登革热病毒持续感染的细胞中,查到的抗原为1型登革热病毒,此种细胞对3型登革热病毒的再感染有明显的干扰。此种干扰作用,在1型登革热病毒感染后8小时开始产生,此
Using indirect immunofluorescence with monoclonal antibody against dengue virus, we studied the type-1 dengue virus-infected C. albopictus C6 / 36 cells and the type-3 dengue virus reinfection. After 3 days of infection of normal cells with type 3 dengue virus, the cells containing type 3 dengue virus antigens were above 90%. However, cells infected with type 1 dengue virus were infected with type 3 dengue virus. As a result, only 0.1 to 1% of cells were infected with type 3 dengue virus antigen. Type 1 dengue virus persistently infected cells were stained with monoclonal antibodies against dengue virus type 1-4 before and after re-infection with type 3 dengue virus, and more than 90% of the cells contained fluorescein. Among the persistent infections with dengue virus type 1, the antigen found was dengue virus type 1, which clearly interfered with the re-infection of type 3 dengue virus. This interference, which starts at 8 hours after infection with type 1 dengue virus, occurs