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建立了良恶性胸腔积液样品中低沸点挥发性有机物(VOCs)的顶空固相微萃取-冷阱聚焦-气相色谱质谱联用(SPME-Cryo Trap-GC/MS)分析方法。应用冷阱聚焦技术后,胸腔积液中低沸点的VOCs得到了很好的分离。对26例样品(10例恶性和16例良性样品)进行了分析,得到76个挥发性物质。以环戊酮为内标求得其中检出频率较高的27种物质的相对峰面积,并对其在恶性组和良性组中的平均值进行计算与比较。丙酮、环己醇、环己酮和2-乙基-1-己醇这4个物质在恶性组中的峰面积平均值明显高于良性组,表现出较大差异。方法适于良恶性胸腔积液中挥发性有机物的比较分析,为肺癌潜在标志物的筛选提供了参考。
A method for the determination of low boiling point volatile organic compounds (VOCs) in benign and malignant pleural effusion samples by headspace solid-phase microextraction coupled with cold trap-gas chromatography-mass spectrometry (SPME-Cryo Trap-GC / MS) was developed. After using cold trap focusing technology, low boiling point VOCs in pleural effusions are well separated. Twenty-six samples (10 malignant and 16 benign) were analyzed and 76 volatile compounds were obtained. The relative peak area of 27 kinds of substances with high detection frequency was obtained with cyclopentanone as internal standard, and the average value of 27 kinds of substances in malignant group and benign group was calculated and compared. The average area of peak area of acetone, cyclohexanol, cyclohexanone and 2-ethyl-1-hexanol in malignant group was significantly higher than that in benign group, showing a big difference. The method is suitable for the comparative analysis of volatile organic compounds in benign and malignant pleural effusions and provides a reference for the screening of potential markers of lung cancer.