Monodispersed silica microspheres with diameter of 353nm were assembled into photonic crystal in ethanol colloidal suspensions of varied silica volume fraction at different temperature and humidity by means of controllable vertical deposition method. The surface morphology and optical properties were studied by SEM and UV-Vis-NIR. It was found that the high-quality silica colloidal photonic crystals were obtained from ethanol solutions with environment temperature between 45℃ and 55℃, humidity between 66% and 76%, the volume fraction of microspheres is between 0.8% and 1.5%. The ordered close-packed photonic crystal fabricated by controllable vertical deposition method had the two photonic bandgaps in the visible light band and near infrared band. Monodispersed silica microspheres with diameter of 353nm were assembled into photonic crystal in ethanol colloidal suspensions of varied silica volume fraction at different temperature and humidity by means of controllablelable vertical deposition method. The surface morphology and optical properties were studied by SEM and UV-Vis -NIR. It was found that the high-quality silica colloidal photonic crystals were obtained from ethanol solutions with ambient temperature between 45 ° C and 55 ° C, humidity between 66% and 76%, the volume fraction of microspheres is between 0.8% and 1.5%. The ordered close-packed photonic crystal fabricated by controllable vertical deposition method had the two photonic bandgaps in the visible light band and near infrared band.