目的 :本文研究了新鲜分离的小鼠脾树突状细胞 (DCs) ,在其未成熟阶段加入MCA38肿瘤抗原 ,使其对抗原加工处理而制成DCs疫苗对同基因型小鼠的抑瘤作用。方法 :通过皮下接种 (A组 ) ,静脉注射 (B组 ) ,腹腔接种 (C组 )DCs疫苗方法研究其抑瘤作用。结果 :经统计学分析表明 ,DCs疫苗对肿瘤的生长具有显著的抑制作用 (P <0 .0 0 1) ;三种接种方法对肿瘤的生长的抑制作用无明显差异 (P>0 .0 5)。结论 :DCs疫苗具有显著的抑瘤作用 ,皮下接种方法接种量少 ,方法简单、安全而且副作用小 ,具有更宽广的临床开发应用前景 OBJECTIVE: To study the effect of freshly isolated mouse spleen dendritic cells (DCs) on the tumor suppressive effect of MCA38 tumor antigen in the immature phase, and to prepare DCs for antigen treatment. . METHODS: The anti-tumor effect of DCs was studied by subcutaneous (Group A), intravenous (Group B), and intraperitoneal (Group C) DCs vaccines. Results: Statistical analysis showed that DCs vaccine had a significant inhibitory effect on tumor growth (P < 0.01); there was no significant difference in the inhibitory effect of the three inoculation methods on tumor growth (P> 0.05). ). Conclusion: The DCs vaccine has significant anti-tumor effect. The subcutaneous inoculation method has less inoculation, the method is simple, safe and has little side effects. It has a broader clinical development and application prospects.