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背景:神经细胞黏附分子L1对神经细胞的发生、发育和神经-神经黏附发挥重要作用,然而L1对细胞突起和功能性突触形成的影响是不清楚的。目的:探讨神经细胞黏附分子L1在神经细胞突起生长和功能性突触形成中的作用。设计:完全随机对照实验研究。地点和材料:地点为广西中医学院神经科学研究所。材料为NG108-15神经细胞株、L1cDNA、抗L1抗体:由日本金泽大学神经物性部门提供。方法:在体外细胞培养,用脂质转染剂将黏附分子L1cDNA表达到NG108-15细胞,用光学显微镜观察细胞形态和电生理学检查技术测定细胞-肌突触的突触后膜电位变化。主要观察指标:细胞突起指数、突触形成率、微小终板电位的频率。结果:以分化剂cAMP处理4d后,L1-转染组有突起分叉的细胞占细胞总数的百分数为(31±8)%,明显高于非转染组的(13±2)%或Mock-转染组的(15±5)%(P<0.001)。L1-转染组的细胞突起平均长度为(142.5±12.3)μm,明显高于非转染组的(94.2±12.3)μm或Mock-转染组的(86.8±6.7)μm(P<0.05)。L1转染组功能性突触的形成率为(58.0±11.5)%,也高于非转染组的(36.7±0.83)%或Mock-转染组的(39.2±0.84)%(P<0.001)。但突触后膜电位的频率在3组之间差异无显著性意义(P>0.05)。结论:L1在NG108-15细胞的高度表达提高cAMP诱发的神经细胞突?
BACKGROUND: Nerve cell adhesion molecule L1 plays an important role in the occurrence, development and neuro-neuronal adhesion of neural cells. However, the effect of L1 on the formation of cellular processes and functional synapses is unclear. Objective: To investigate the role of neural cell adhesion molecule L1 in neurite outgrowth and functional synapse formation. Design: Complete randomized controlled experimental study. Location and materials: The location is Institute of Neuroscience, Guangxi College of Traditional Chinese Medicine. Materials for NG108-15 neural cell lines, L1 cDNA, anti-L1 antibody: provided by the Neurology Department of Kanazawa University in Japan. Methods: L1cDNA was expressed in NG108-15 cells by lipofectamine in vitro. The changes of postsynaptic membrane potential were detected by light microscope and morphological changes and electrophysiological examination. MAIN OUTCOME MEASURES: Cell protrusion index, synapse formation rate, frequency of micro-plateau potential. RESULTS: After treated with cAMP for 4 days, the percentage of cells with protruding bifurcation in the L1-transfected group was (31 ± 8)%, which was significantly higher than that of the untransfected group (13 ± 2)% or Mock - (15 ± 5)% of transfection group (P <0.001). The average length of the cell protrusions in the L1-transfected group was (142.5 ± 12.3) μm, significantly higher than that in the non-transfected group (94.2 ± 12.3 μm) or the Mock-transfected group (86.8 ± 6.7) μm (P <0.05) . The formation rate of functional synapses in L1 transfected group was (58.0 ± 11.5)%, higher than that in non-transfected group (36.7 ± 0.83)% or Mock-transfected group (39.2 ± 0.84)% ). However, there was no significant difference in the frequency of postsynaptic membrane potential between the three groups (P> 0.05). Conclusion: The high expression of L1 in NG108-15 cells enhances cAMP-induced neuronal processes.