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目的探讨斑点金免疫渗滤法(DIGFA)检测血吸虫抗体的应用价值。方法采用斑点金免疫渗滤法(DIGFA)与ELISA法对1088份不同血清标本作平行检测,统计分析检测结果。结果DIGFA法检测急性、慢性、晚期血吸虫病和水牛血吸虫病的敏感性分别为100%、98.30%、82.86%和98.71%;特异性为97.52%～100%;检测肺吸虫病和蛔虫病的交叉反应率分别为6.06%和2.78%,检测结果与ELISA相似。结论DIGFA法作为一种血吸虫病快速诊断方法,具有较高的敏感性和特异性,且操作简便快速,不需特殊仪器设备,有着较好的应用前景。
Objective To investigate the application value of DIGFA in detecting schistosome antibody. Methods 1088 serum samples were tested in parallel by dot immunogold filtration assay (DIGFA) and ELISA, and the results were statistically analyzed. Results The sensitivity of DIGFA in the detection of acute, chronic, advanced schistosomiasis and buffalos were 100%, 98.30%, 82.86% and 98.71%, respectively. The specificity was 97.52% -100%. The detection of paragonimiasis and ascariasis The response rates were 6.06% and 2.78%, respectively, and the results were similar to ELISA. Conclusion DIGFA method as a rapid diagnostic method for schistosomiasis, with high sensitivity and specificity, and the operation is simple and fast, without special equipment, has a good prospect.