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目的:探讨生脉注射液对不同周龄大鼠睾丸扭转复位后睾丸损伤影响的差异。方法:3、6、12周龄健康雄性SD大鼠各16只,随机分成睾丸扭转复位+注射生脉注射液组(实验组)和睾丸扭转复位+注射生理盐水组(对照组),每组8只,建立睾丸扭转动物模型(左侧阴囊切开,绕精索顺时针扭转睾丸720°2h),并于手术后24h处死,测定各组大鼠睾丸组织内总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量。结果:与各自对照组比较,3、6周实验组大鼠左侧睾丸组织中T-AOC、SOD活性和MDA含量均无显著性改变(P>0.05);12周实验组大鼠左侧睾丸组织中SOD、T-AOC明显升高,而MDA含量显著降低,差异均具有显著性(P<0.05)。结论:生脉注射液对睾丸扭转复位后的缺血再灌注急性损伤有保护作用,但对不同周龄大鼠的再灌注损伤保护作用不同,存在年龄相关性差异,对较大周龄大鼠的急性保护作用较为明显。
Objective: To investigate the effect of Shengmai injection on the testicular damage after testicular torsion in different age groups. Methods: Thirty six male Sprague-Dawley rats were randomly divided into testicular torsion reduction + injection Shengmai injection group (experimental group) and testicular torsion reduction + saline injection group (control group) (T-AOC group). T-AOC (T-AOC) model was established in all the rats with testicular torsion. The left scrotum was incised and the spermatic cord was wriggled around the spermatic cord for 720 ° 2 h clockwise. ), Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. RESULTS: Compared with the control group, there was no significant change in T-AOC, SOD activity and MDA content in the left testes of the experimental groups at 3 and 6 weeks (P> 0.05). In the left experimental testes Tissue SOD, T-AOC increased significantly, while the MDA content was significantly reduced, the difference was significant (P <0.05). Conclusion: Shengmai injection has a protective effect on acute ischemia-reperfusion injury after testicular torsion reduction. However, the protective effects of Shengmai injection on reperfusion injury in rats of different weeks have different age-related differences. The acute protective effect is more obvious.