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直接观察体外培养人脐静脉内皮细胞（HUVEC）上清液中血管内皮舒张因子（EDRF／NO）的释放变化及对血小板聚集的抑制作用。发现：（1）缓激肽（BK）或细菌脂多糖（LPS）能刺激（EDRF／NO增加到原来的2倍，并且以孵育第1h增长最多。（2）L-精氨酸（L－Arginine）作为EDRF／NO合成原料，可以提高HUVEC上清液中EDRF／NO量，但是EDRF／NO合成酶抑制剂L－NG－nitro－arginine（L－NNA）与L－Arginine同时存在时则降低这个合成量。（3）经消炎痛处理过并含有EDRF／NO的HUVEC上清液对血小板有抑制作用、这种抑制作用由于L－NNA的存在而减弱。
To directly observe the release of vascular endothelial relaxing factor (EDRF / NO) in supernatants of human umbilical vein endothelial cells (HUVEC) in vitro and the inhibitory effect on platelet aggregation. The results showed that: (1) EDRF / NO increased by 2 folds and increased most at 1 hour after incubation with BK or LPS; (2) L-arginine (L- Arginine as an EDRF / NO synthetic material can increase the amount of EDRF / NO in the supernatant of HUVEC. However, the EDRF / NO synthase inhibitor L-NG-nitro-arginine (L-NNA) (3) HUVEC supernatants treated with indomethacin and containing EDRF / NO have an inhibitory effect on platelets, which is attenuated by the presence of L-NNA.