一、蜂囊菌的液体培养基培养实验方法从病死蜂幼虫中分离鉴定的蜂囊菌孢子在Sabouraud氏琼脂培养基上于30℃及适宜湿度下培养,形成子实体后刮下菌落置研砵中,加入ycc汤汁研磨,使其子囊释放,再用Showa型寄生虫卵检验仪(230目)过滤,离心沉淀后,用适宜浓度的ycc汤稀释沉淀,制成子囊悬液。将该悬液接种于特制的花粉、糖、内 First, the liquid medium culture method of the insect of bee cysts The isolation and identification of the insects of the insects from the dead bee larvae on the Sabouraud agar medium at 30 °C and suitable humidity, the formation of fruiting bodies after the scraping colonies set In the process, ycc soup was added to grind it to release the ascoses, and then filtered using a Showa parasite egg tester (230 mesh). After centrifugation, the precipitate was diluted with a suitable concentration of ycc soup to prepare an ascus suspension. The suspension is inoculated into special pollen, sugar,