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本文用经照射的旋毛虫新生幼虫感染的骨骼肌,研究其对细胞周期所起的作用及其在感染细胞中的生长发育。 新生幼虫接受~(60)Co(2.9krad/min)的照射。将照射幼虫悬浮于Delbecco的改良Ea-gle培养基(DMEM)(1×10~6个幼虫/ml)中,然后注入小鼠腓肠肌中,感染后定期取组织观察并分离幼虫,以评价其活力并测量长度。测量长度时首先将幼虫置65℃加热5min以
In this paper, the infected skeletal muscle of Trichinella spiralis neonatal larvae was used to study its role in cell cycle and its growth in infected cells. Neonatal larvae receive ~ (60) Co (2.9krad / min) irradiation. The irradiated larvae were suspended in Delbecco’s modified Ea-gle medium (DMEM) (1 × 10 6 larvae / ml) and then injected into the gastrocnemius muscle of the mice. After the infection, the larvae were periodically taken for observation and the larvae were separated to evaluate their viability And measure the length. When measuring the length of the first larvae set 65 ℃ heating 5min