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目的 检测胃癌组织中 p16基因及启动子甲基化状态和p16蛋白表达情况。 方法 选择p16基因及启动子区域 ,用PCR SSCP、MSP(甲基化特异的PCR)法、测序和免疫组化等方法对 10 0例胃癌患者的癌组织和癌旁组织进行检测。结果 71%的病例 p16表达阴性 ,5 4%的病例具有p16基因启动子区的高甲基化 ,5 0 %的病例同时有 p16表达阴性和 p16基因启动子区的高甲基化 ,无突变和纯合缺失检出。结论 提示p16基因启动子区域高甲基化是胃癌中 p16基因失活的主要原因。
Objective To detect the p16 gene and promoter methylation status and p16 protein expression in gastric cancer. Methods The p16 gene and its promoter region were selected. PCR and SSCP, MSP (methylation-specific PCR) method, sequencing and immunohistochemistry were used to detect the cancer tissues and paracancerous tissues of 100 patients with gastric cancer. RESULTS: p16 was negative in 71% of cases, hypermethylation of the promoter region of p16 gene in 54% of cases, and negative p16 expression and hypermethylation of the promoter region of p16 gene in 50% of cases. No mutation and homozygous deletion Check out. Conclusions The hypermethylation of p16 gene promoter region is the main reason for the inactivation of p16 gene in gastric cancer.