目的　建立测定血清中利福平浓度的高效液相色谱法 ,并应用于临床。方法　采用Nova -pakC18色谱柱 (4 μm ,15 0nm× 3.9mm) ,以甲醇 - 0 .0 2mol·L-1醋酸钠 (6 2∶4 0 )为流动相 ,紫外检测波长 334nm ,测定口服利福平 4d以上的患者血清中利福平的浓度。结果　血清中利福平在 0 .5～ 16 .0 μg·ml-1范围内浓度与峰面积线性关系良好 (r=0 .9992 ) ,平均回收率为99 .4 3% ,日内、日间RSD <5 .0 %。结论　所用方法准确、灵敏 ,用血量少 ,适用于治疗药物监测 ,为临床用药提供数据参考 Objective To establish a HPLC method for the determination of rifampicin in serum and apply it in clinic. Methods Nova -pak C18 column (4 μm, 150 nm × 3.9 mm) was used with methanol-0.02 mol·L-1 sodium acetate (6:40) as the mobile phase. The UV detection wavelength was 334 nm. The level of rifampicin in the sera of patients over 4 days of Fuping. Results The concentration of rifampicin in serum ranged from 0.5 to 16.0 μg · ml-1 and the peak area was linear (r = 0.9992). The average recovery was 99.43%. The intra-day and inter-day RSD <5 .0%. Conclusion The method used is accurate and sensitive, with less blood, suitable for the monitoring of therapeutic drugs and data reference for clinical medication