论文部分内容阅读
本文报道运用巢式多聚酶链反应,ELISA等方法检测我国原发性肝癌、癌旁组织和外周血中HCV-RNA,抗-HCV、同时监测了HBVAg,所用引物位于HCV基因5’-端非编码区域。结果76例肝癌病人血清标本7例抗-HCV阳性,(7/76,9.2％)10例HCV-RNA阳性(10/76、13.2％);34例肝癌组织中4例HCV-RNA阳性(4/34,11.0％)。1例抗-HCV阴性的病人肝癌及癌旁组织中检测到HCV-RNA提示我国的HCC发病与HCV感染似有一定关系。本组检测到10例HCV-RNA阳性,其中4例(40％)系第二步扩增后检出,说明巢式PCR确可显著提高HCV-RNA的检出率,单纯用C100-3抗体检测有可能低估HCC中HCV的感染率。本研究发现HCVAg阳性的HCC中HBsAg检出率高于HCVAg阴性HCC者(P<0.05)。说明在我国HCC的发生中,HCV可单独,但更常与HBV共同作用。
This article reports the use of nested polymerase chain reaction, ELISA and other methods to detect HCV-RNA, anti-HCV, and HBVAg in primary liver cancer, adjacent tissues and peripheral blood. The primers used are located in the 5’-end uncoded HCV gene. area. Results Serum samples from 76 patients with hepatocellular carcinoma were positive for anti-HCV, (7/76, 9.2%) were positive for HCV-RNA (10/76, 13.2%); 4 of 34 cases of hepatocellular carcinoma were positive for HCV-RNA (4 /34,11.0%). One case of anti-HCV-negative patients with hepatocellular carcinoma and adjacent tissues detected HCV-RNA suggests that the incidence of HCC in China seems to have a certain relationship with HCV infection. 10 HCV-RNA positives were detected in this group, 4 of which (40%) were detected after the second step of amplification, indicating that nested PCR can significantly increase the detection rate of HCV-RNA, using only C100-3 antibody The test may underestimate the HCV infection rate in HCC. This study found that the detection rate of HBsAg in HCVAg positive HCC was higher than that in HCVAg negative HCC (P<0.05). It shows that in the occurrence of HCC in China, HCV can be isolated, but it often interacts with HBV.