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谷胱甘肽转移酶(GST)是一组具有多种生理功能的二聚体蛋白质,它广泛存在于哺乳动物的各种组织中。GST能催化许多疏水亲电子物质与谷胱甘肽结合,还可以非酶形式结合,将体内毒性物质和亲脂性化合物排出体外。因此,GST在解毒和抗脂质过氧化反应中起重要作用。近年来发现GST与化学致癌有关,在肿瘤发生、发展和转归中有重要意义,是一种肿瘤新型标志酶。本组采用生物素-亲和素-酶联免疫吸附法(BA-ELISA)对78例消化道肿瘤患者术前、术后血清总GST的变化作动态观察,结果表明,肿瘤患者的术前血清总GST为1.07±0.61mg/ml,明显高于对照的0.41±0.11ng/ml(P<0.05),而原发性肝癌病人术前血清GST明显高于胃癌、结、直肠癌病人,其它组间病人的血清GST差异不大。可见,GST在消化道肿瘤时有一定表达,可作为消化道肿瘤的一个标志酶,尤其对诊断肝脏病变有一定价值。但对判断消化道肿瘤部位意义不大。肿瘤病人血清GST升高可能来源于肿瘤细胞本身。手术切除肿瘤后,血清GST水平迅速下降,术后14天已降至正常范围,因此GST也可作为判断肿瘤治疗效果和预后的指标之一。
Glutathione transferase (GST) is a group of dimeric proteins with various physiological functions that are widely present in various mammalian tissues. GST can catalyze the binding of many hydrophobic electrophilic substances to glutathione and can also be combined in a non-enzymatic form, expelling in vivo toxic substances and lipophilic compounds from the body. Therefore, GST plays an important role in detoxification and anti-lipid peroxidation. In recent years, it has been found that GST is involved in chemical carcinogenesis and has important significance in tumorigenesis, development, and prognosis. It is a novel tumor marker enzyme. This group used biotin-avidin-enzyme-linked immunosorbent assay (BA-ELISA) to dynamically observe changes in serum total GST before and after surgery in 78 patients with digestive tract cancer. The results showed that preoperative serum of patients with cancer The total GST was 1.07±0.61 mg/ml, which was significantly higher than that of the control (0.41±0.11 ng/ml) (P<0.05). The preoperative serum GST in patients with primary liver cancer was significantly higher than that in patients with gastric cancer, colorectal cancer, and other groups. There was no significant difference in serum GST between patients. Visible, GST has a certain expression in the digestive tract tumors, can be used as a marker enzyme of digestive tract tumors, especially for the diagnosis of liver lesions have a certain value. However, it is of little significance to determine the location of the digestive tract tumor. The elevated serum GST in cancer patients may originate from the tumor cells themselves. After surgical removal of the tumor, the serum GST level rapidly decreased and was reduced to the normal range within 14 days after the operation. Therefore, GST can also be used as one of the indexes to judge the therapeutic effect and prognosis of the tumor.