论文部分内容阅读
目的：用培养ＳＤ仔鼠心肌细胞为模型，观察自发性高血压大鼠（ＳＨＲ）肥厚心肌匀浆上清提取物对培养心肌细胞核酸合成、蛋白质含量和细胞体积的影响及钙通道阻滞剂维拉帕米对该提取物作用的干预．方法：参照Ｓｅｎ法制备心肌匀浆提取液，心肌细胞培养２４ｈ后加入ＳＨＲ心肌提取物，测定培养心肌细胞３Ｈ－ＵＲ参入率，细胞蛋白质含量和心肌细胞体积．结果：与对照大鼠相比，ＳＨＲ肥厚心肌匀浆提取物可促使培养心肌３Ｈ－ＵＲ参入率［（３１６８±６６２）ｃｐｍ对（２３８４±６０１）ｃｐｍ］，心肌细胞平均蛋白质含量［（１１８２．５±１０４．１）ｐｇ／ｃｅｌ对（９８６．６±８１．３）ｐｇ／ｃｅｌ］，心肌细胞平均体积［（１７２４．９±２８．２）μｍ３／ｃｅｌ对（１４５８．３±２７．６）μｍ３／ｃｅｌ］均显著增加（Ｐ均＜０．０５）．维拉帕米可部分抑制该提取物的作用使培养心肌细胞３Ｈ－ＵＲ参入率显著降低［（２７４５±５８２）ｃｐｍ对（３１６８±６６２）ｃｐｍ，Ｐ＜０．０５］，平均蛋白质含量显著降低［（１０３７．８±８４．３）ｐｇ／ｃｅｌ对（１１８２．５±１０４．１）ｐｇ／ｃｅｌ，Ｐ＜０．０５］，细胞体积显著缩小［（１５２１．４±２３．９）μｍ３／
OBJECTIVE: To observe the effects of hypertrophic myocardium homogenate supernatant extract on nucleic acid synthesis, protein content and cell volume in cultured myocardial cells of neonatal SD rats using cultured cardiomyocytes of neonatal SD rats as model and calcium channel blockers Verapamil interventions on the role of the extract. Methods: The myocardial homogenate extract was prepared by reference to the Sen method. After myocardial cells were cultured for 24 hours, the myocardial extracts of SHR were added to determine the 3H-UR incorporation, protein content and cardiomyocyte volume in cultured cardiomyocytes. Results: Compared with the control rats, SHR hypertrophy myocardial homogenate extract could promote the 3H-UR incorporation rate [(3168 ± 662) cpm vs (2384 ± 601) cpm] and the average myocardial protein content [(1182) 5 ± 104.1) pg / cel pair (986.6 ± 81.3) pg / cel], mean myocardial cell volume [(1724.9 ± 28.2) μm3 / cel vs ) Μm3 / cel] were significantly increased (all P <0.05). The effect of verapamil on the inhibition of 3H-UR incorporation in cultured cardiomyocytes was significantly lower than that of verapamil [(2745 ± 582) cpm vs (3168 ± 662) cpm, P <0.05], and the average protein content was significantly decreased The cell volume was significantly reduced [(1037.8 ± 84.3) pg / cel vs (1182.5 ± 104.1) pg / cel, P <