论文部分内容阅读
将瓜果腐霉进行液体发酵,其菌丝用等体积乙酸乙酯和甲醇进行依次萃取,甲醇萃取液旋转蒸发去溶剂后进行硅胶柱层析,以乙酸乙酯和石油醚(V/V=3:1和V/V=2:1)的混合液进行梯度洗脱,每50 mL收集为一个馏分,共收集到40个馏分。生物测定结果表明,以乙酸乙酯和石油醚(V/V=2:1)洗脱得到的馏分21 24对供试杂草马唐表现出了较强的活性,其对马唐的生长抑制作用均为4级。合并馏分21 24,以乙酸乙酯和石油醚(V/V=2:1)的混合液为展开剂进行等度洗脱,每50 mL收集为一个馏分,共收集到20个馏分。生物测定结果表明,馏分3对马唐有较强的抑制活性。HPLC分析发现,该馏分主要含有3个组分,其保留时间分别为12.7、14.0和30.5 min。
The liquid was fermented by Pythium melilotoides, the mycelium was extracted by the same volume of ethyl acetate and methanol in turn, the methanol extract was rotary evaporated to remove the solvent and silica gel column chromatography with ethyl acetate and petroleum ether (V / V = 3: 1 and V / V = 2: 1) was used for gradient elution. A total of 40 fractions were collected every 50 mL. The results of bioassay showed that fraction 21 24 eluted with ethyl acetate and petroleum ether (V / V = 2: 1) showed strong activity on Crabgrass, which inhibited the growth of Crabgrass The role of all four. The combined fractions 21 24 were isocratically eluted with a mixture of ethyl acetate and petroleum ether (V / V = 2: 1) as eluent. One fraction was collected per 50 mL and a total of 20 fractions were collected. Bioassay results showed that fraction 3 had a strong inhibitory activity on Crabgrass. HPLC analysis found that the fraction mainly contains three components, the retention time was 12.7,14.0 and 30.5 min.