目的　观察通脉宁胶囊不同剂量药物血清对溶血磷脂酰胆碱 (LPC)诱导的血管平滑肌细胞增殖活性及细胞周期的影响。方法　组织贴块法进行血管平滑肌细胞 (VSMC)培养 ,应用血清药理学的方法 ,LPC10 -8mol L作为刺激因子 ,将通脉宁胶囊分为大、中、小 3个剂量组。MTT法测定细胞增殖活性 ,流式细胞仪检测细胞周期。结果　通脉宁大、中、小剂量组可明显降低细胞的OD值呈剂量依赖性地抑制LPC诱导的VSMC增殖。明显降低细胞的S期数目百分比 ,增加G0 G1期数目百分比 ,使VSMC阻滞在G0 G1期 ,抑制细胞进入S期。结论　从细胞分子水平说明通脉宁胶囊主要是通过影响细胞内DNA合成 ,减少有丝分裂 ,增加G0 G1期数目百分比 ,减少S期数目百分比而发挥抑制VSMC增殖作用。 Objective To observe the effect of different doses of Tongmaining Capsule on serum lysolecithin (LPC)-induced vascular smooth muscle cell proliferation and cell cycle. Methods Tissue culture method was used to culture vascular smooth muscle cells (VSMCs). Serum pharmacological methods were used. LPC10 -8 mol L was used as the stimulating factor. Tongmaining capsules were divided into large, medium and small dose groups. The cell proliferation activity was determined by MTT assay and the cell cycle was measured by flow cytometry. Results The large, medium and low doses of Tongmailing could significantly reduce the OD value of cells in a dose-dependent manner to inhibit LPC-induced VSMC proliferation. Significantly reduce the percentage of cells in the S phase, increase the percentage of G0 G1 phase, so that VSMC arrest in the G0 G1 phase, inhibition of cells into the S phase. Conclusion From the cell molecular level, Tongmaining capsules can be used to inhibit the proliferation of VSMC by affecting intracellular DNA synthesis, reducing mitosis, increasing the percentage of G0 G1 phase, and reducing the percentage of S phase phase.