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采用免疫组织化学方法及放射配体结合分析法 ,观察清开灵及侧脑室注射c fos反义寡核苷酸阻断c fos基因表达后脑组织蛋白激酶C(PKC)表达量和Glu的N_甲基_D_天门冬氨酸 (NM DA)受体的数目、亲和力变化的影响。结果 :清开灵可有效地抑制Glu所致的脑组织c fos蛋白及PKC蛋白的表达增强 ,并下调Glu的NMDA受体数目 ;而c fos反义及正义寡核苷酸对Glu的NMDA受体数目及Glu所致的脑组织PKC蛋白表达增多均无明显影响。结论 :清开灵抗Glu神经毒性的脑保护作用的机制与其抑制c fos和PKC蛋白基因的表达及下调NMDA受体数目有关
Immunohistochemistry and radioligand binding assays were used to observe the expression of protein kinase C (PKC) and Glu of the brain tissue after blocking the expression of cfos gene by injection of c fos antisense oligonucleotide into Qing Kai Ling and lateral ventricle. Effects of changes in the number and affinity of methyl-D-aspartate (NM DA) receptors. RESULTS: Qingkailing can effectively inhibit the expression of cfos protein and PKC protein in brain tissue induced by Glu, and down-regulate the number of NMDA receptors in Glu; while the c-fos antisense and sense oligodeoxynucleotides inhibit NMDA in Glu. The number of brain tissues and the increase of PKC protein expression in the brain caused by Glu had no significant effect. Conclusion : The mechanism of brain protective effect of Qingkailing against Glu neurotoxicity is related to its inhibition of c fos and PKC protein gene expression and down-regulation of NMDA receptors