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目的:观察口服葡萄糖负荷对小鼠小肠组织网膜素基因表达的影响及网膜素对C2C12肌管细胞胰岛素敏感性的影响,并进一步探讨其机制。方法:半定量逆转录聚合酶链反应(RT-PCR)技术检测小鼠小肠组织网膜素mRNA的表达;葡萄糖转运实验观察网膜素对C2C12肌管细胞胰岛素敏感性的影响;Western blot检测Akt(Ser473)的磷酸化水平。结果:口服葡萄糖负荷30min后小鼠小肠组织网膜素基因表达显著减低(P<0.05),而60min后恢复至负荷前水平;葡萄糖转运实验发现:网膜素作用10min对C2C12肌管细胞基础葡萄糖转运无影响,但显著增加了胰岛素刺激的葡萄糖转运(P<0.05);Western Blot发现:网膜素和AICAR均显著增加了C2C12肌管细胞Ak(tSer473)的磷酸化水平(P<0.05)。结论:网膜素作为一种胃肠激素还受到葡萄糖负荷的调节,在C2C12肌管细胞,网膜素可通过增加Akt的磷酸化发挥胰岛素增敏作用。
OBJECTIVE: To observe the effects of oral glucose load on the expression of retinal genes in small intestine and the effects of retinal insulin on the insulin sensitivity of C2C12 myotubes, and to explore its mechanism. Methods: The expression of retinal mRNA in small intestine of mice was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The effect of retinal insulin on the insulin sensitivity of C2C12 myotubes was observed by glucose transport assay. The expression of Akt (Ser473) phosphorylation levels. Results: The expression of retinal genes in the small intestine of mice was significantly reduced (P <0.05) 30 min after oral glucose administration, and returned to pre-load level after 60 min. Glucose transport assay showed that the retinal function of 10 min on the base glucose of C2C12 myotubes (P <0.05). Western Blot showed that both retinal and AICAR significantly increased phosphorylation of Akt (C2C12) myotubes Akt (tSer473) (P <0.05). CONCLUSION: Overactive retinal hormones are also regulated by glucose load. In C2C12 myotubes, retinol can exert insulin sensitization by increasing Akt phosphorylation.