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目的 探讨内蒙古汉族儿童 HL A- DQA1等位基因与过敏性紫癜 (anaphylactoid purpura,AP)的遗传易感性及其与临床特点的关系。方法 用聚合酶链反应 -序列特异性引物技术 ,对 70例内蒙古汉族儿童 AP和 90名健康儿童 HL A- DQA1等位基因进行对比分析。结果 (1)病例组 DQA1* 0 30 1基因频率为 33.4% ,明显高于对照组 (10 .6 % ) (P<0 .0 1) ;DQA1* 0 30 2基因频率为 6 .7% ,明显低于对照组(19% ) (P<0 .0 1)。 (2 )单纯皮肤紫癜病例组与对照组 DQA1* 0 30 1和 0 30 2基因频率比较 ,差异无显著性(P>0 .0 5 ) ;伴有胃肠、关节、肾脏损害病例 DQA1* 0 30 1基因频率分别为 2 6 .7%、2 8.5 %和 2 9.3% ,均明显高于对照组 (10 .6 % ) (P均 <0 .0 1) ;而 DQA1* 0 30 2基因频率分别为 3.9%、5 .7%和 9.6 % ,分别与对照组 (19% )比较 ,肾脏损害组差异无显著性 (P>0 .0 5 ) ,胃肠和关节损害组均明显降低 (P均 <0 .0 1)。结论HL A- DQA1* 0 30 1等位基因可能是内蒙古汉族儿童 AP发病单体型中一个遗传易感基因 ,具此基因者可能更易出现胃肠、关节和肾脏损害 ;而 DQA1* 0 30 2等位基因可能为其遗传保护基因 ,对出现胃肠、关节损害可能有拮抗作用。
Objective To investigate the genetic susceptibility of HL-DQA1 alleles and anaphylactoid purpura (AP) in Han children in Inner Mongolia and its relationship with clinical features. Methods The alleles of HLA-DQA1 in 70 Chinese Han children and 90 healthy children in Inner Mongolia were analyzed by polymerase chain reaction-sequence-specific primers. Results The frequency of DQA1 * 0301 gene in case group was 33.4%, significantly higher than that in control group (10.6%) (P <0.01). The frequency of DQA1 * 0302 gene was 6.7% Significantly lower than the control group (19%) (P <0.01). (2) The frequencies of DQA1 * 0 30 1 and 0 30 2 genes between the pure cutaneous Purpura cases and the control group were not significantly different (P> 0.05). The cases with gastrointestinal, joint and kidney damage were DQA1 * 0 30 1 gene frequencies were 26.7%, 28.5% and 29.3%, respectively, which were significantly higher than those in the control group (10.6%) (P <0.01). However, the frequency of DQA1 * 0302 gene (3.9%, 5.7% and 9.6% respectively). Compared with the control group (19%), there was no significant difference in the kidney damage group (P> 0.05) All <0.01). CONCLUSION: HL-DQA1 * 0301 allele may be a genetic susceptibility gene in the haplotypes of AP in Han children in Inner Mongolia. Patients with this gene may be more likely to have gastrointestinal, joint and kidney damage. However, DQA1 * 0302 Alleles may be their genetic protection of genes, gastrointestinal, joint damage may have antagonistic effects.