论文部分内容阅读
目的探讨苯妥英钠（Ｄｉｐｈｅｎｙｌｈｙｄａｎｔｏｉｎ，ＤＰＨ）在急性缺氧条件下的脑保护作用。方法给大鼠应用ＤＰＨ，用荧光分光法检测其在低压舱模拟升空７．５ｋｍ后１ｈ、２ｈ的脑匀浆单胺递质含量，同时观察脑细胞形态学改变。结果ＤＰＨ实验组在缺氧１ｈ及２ｈ时脑多巴胺和５－羟色胺含量升高（Ｐ＜０．０５），而脑去甲肾上腺素在缺氧１ｈ时降低（Ｐ＜０．０５），缺氧２ｈ恢复正常。电镜形态学观察，低压缺氧条件下对照组大鼠脑细胞水肿，线粒体嵴严重缺失，细胞核明显变形；应用ＤＰＨ的实验组细胞形态基本正常。结论给大鼠应用苯妥英钠后，在急性缺氧条件下，动物脑单胺递质含量的变化可能与缺氧适应或耐受的形成有关。
Objective To investigate the protective effect of diphenylhydantoin (DPH) on acute cerebral hypoperfusion. Methods DPH was used in rats. The content of monoamine neurotransmitters in brain homogenate was measured by fluorescence spectrophotometry after simulated 7.5 km lift-off in the low-pressure cabin. The morphological changes of brain cells were also observed. Results The levels of dopamine and 5-hydroxytryptamine in the DPH group were significantly increased at 1 h and 2 h (P <0.05), while those in the brain decreased at 1 h (P <0.05) 2h returned to normal. Under the condition of hypobaric hypoxia, the edema and mitochondrial cristae of rats in the control group were severely deficient and the nuclei were obviously deformed. The cell morphology of the experimental group treated with DPH was basically normal. Conclusion After phenytoin is administered to rats, the changes of monoamine neurotransmitters in the brain may be related to the formation of hypoxia adaptation or tolerance in acute hypoxia.