论文部分内容阅读
本文报道已构建的1型单纯疱疹病毒(HSV-1)糖蛋白D(gD1)基因表达质粒pK-TS 153在小鼠中的免疫原性和对HSV-2粘膜攻击的抵抗作用.将DNA疫苗间隔3周肌肉接种体重为18～21g的雌性小鼠3次,每次接种含gDl质粒DNA 200μg的无菌盐水0.1ml,取第3次接种后3周的血清和用PBS冲洗的阴道分泌物,用ELISA测定抗体.对照组为年龄配对的未处理小鼠.结果从免疫的两组Swiss Webster小鼠和一组BALB/c小鼠的血清中均测到HSV特异性IgG,平均滴度为3.5～3.6log_(10).9只Swiss Webster小鼠中有7只阴道分泌物中测到HSV IgG,3组平均滴度分别为1.9、1.6和2.8log_(10).未从3
This article reports the immunogenicity and resistance to HSV-2 mucosal challenge in a mouse model of the HSV-1 glycoprotein D (gD1) gene expression plasmid pK-TS15, Female mice weighing 18-21 g were inoculated intramuscularly three times at 3-week intervals, and 0.1 ml of sterile saline containing 200 μg of gDl plasmid DNA was inoculated each time. Serum of 3 weeks after the third inoculation and vaginal discharges rinsed with PBS , And the antibody was measured by ELISA.The control group was age-matched untreated mice.Results HSV-specific IgG was detected in sera of two immunized Swiss Webster mice and a group of BALB / c mice with an average titer of 3.5 ~ 3.6log_ (10) HSV IgG was detected in 7 vaginal secretions from 9 Swiss Webster mice with average titers of 1.9, 1.6 and 2.8 log 10, respectively