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目的探讨反义肽核酸(phosphono sense peptide nucleic acid,P-PNA)对肺癌SPC-A-1凋亡作用的影响。方法采用肺癌细胞株(SPC-A-1)培养,待细胞为对数生长期时,传代培养30例标本,分为P-PNA组、脂质体组、空白对照组,每组10例,转染前后用RT-PCR,流式细胞仪检测,分别检测端粒酶活性和细胞凋亡百分率。结果转染后P-PNA的端粒酶活性水平有所下降,ATP的浓度明显降低,空白对照组与脂质体组无明显的变化(P<0.05)。P-PNA组肺癌细胞的凋亡率明显增加,与其他两组比较差异显著(P<0.05)。结论 P-PNA在载体运载下,可以进入SPC-A-1细胞线粒体中,通过影响端粒酶的活性阻碍编码基因的转录,进而影响肺癌细胞的合成并诱导其凋亡。
Objective To investigate the effect of P-PNA on apoptosis of SPC-A-1 cells in lung cancer. Methods The lung cancer cell line (SPC-A-1) was cultured. When the cells were in logarithmic growth phase, 30 samples were subcultured and divided into P-PNA group, liposome group and blank control group, RT-PCR and flow cytometry were used to detect the telomerase activity and percentage apoptosis respectively before and after transfection. Results After transfection, the level of P-PNA decreased in telomerase activity and the concentration of ATP decreased obviously. There was no significant difference between the blank control group and the liposome group (P <0.05). P-PNA group apoptosis rate of lung cancer cells increased significantly, compared with the other two groups significant difference (P <0.05). Conclusion P-PNA can enter the mitochondria of SPC-A-1 cells under the carrier and inhibit the transcription of the encoding gene by affecting the activity of telomerase, thereby affecting the synthesis of lung cancer cells and inducing apoptosis.