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目的:探讨化痰祛湿活血方对非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)大鼠肝组织炎症损伤及纤维化的影响。方法:雄性SD大鼠72只,随机分为空白组,模型组,多烯磷脂酰胆碱胶囊对照组,化痰祛湿活血方高(50.4 g/kg)、中(25.2 g/kg)、低剂量(12.6 g/kg)组。采用化学发光法检测化痰祛湿活血方对NASH大鼠谷丙转氨酶(glutamic-pyruvic transaminase,ALT)、谷草转氨酶(millet straw transaminase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、甘油三酯(triglycerides,TG)、胆固醇(cholesterol,TC)、葡萄糖(glucose,GLU)的影响,制作石蜡切片行苏木素-伊红染色(HE)检测化痰祛湿活血方对NASH大鼠肝组织炎症损伤的影响,行淀粉酶预处理-过碘酸雪夫氏染色(DPAS)检测化痰祛湿活血方对NASH大鼠肝组织中库普弗细胞的影响,行马松三色染色(Masson)检测化痰祛湿活血方对NASH大鼠肝组织纤维化的影响。结果:与模型组相比,各用药组ALT、AST、ALP、TG、TC、GLU均有不同程度下降(P<0.05);高剂量组改善最显著(P<0.05);高剂量组在改善NASH大鼠炎症损伤及纤维化方面优于其他用药组(P<0.05)。结论:化痰祛湿活血方能够改善NASH大鼠肝组织炎症及纤维化程度,高剂量组疗效最显著。
Objective: To investigate the effect of Huatan Qushi Huoxue Recipe on liver injury and fibrosis in non-alcoholic steatohepatitis (NASH) rats. Methods: Seventy two male Sprague-Dawley rats were randomly divided into blank group, model group, polyene phosphatidylcholine capsule control group, Phlegm dampness and blood circulation high (50.4 g / kg), medium (25.2 g / kg) Low dose (12.6 g / kg) group. The chemiluminescence method was used to detect the effects of Huatan Qushi Huhuo Recipe on glutamic-pyruvic transaminase (ALT), millet straw transaminase (AST), alkaline phosphatase (ALP), triglyceride Triglycerides (TG), cholesterol (TC) and glucose (GLU) were measured in paraffin sections of rats. The paraffin sections were made and stained with hematoxylin-eosin (HE) The effect of Huatan Huashi Huoxue Recipe on Kupffer cells in liver tissue of NASH rats was detected by amylase pretreatment-periodic acid Schiff’s stain (DPAS) .Masson staining Effect of Phlegm Qushi Huoxue Recipe on Hepatic Fibrosis in NASH Rats. Results: Compared with the model group, the ALT, AST, ALP, TG, TC and GLU of all the groups decreased to different extents (P <0.05); the improvement of the high dose group was the most significant The inflammation and fibrosis of NASH rats were better than other groups (P <0.05). Conclusion: Huatan Qushi Huoxue Prescription can improve liver inflammation and fibrosis in NASH rats. The effect of high-dose group is the most significant.