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观察硒(Se)、环磷酞胺(CY)和白介素-2 (1L-2)单独和联合应用延长荷瘤小鼠生存期效果。实验分7组,每组10只:除对照外,3组为单独应用(Se、CY、IL-2),3组为联合应用(Se+CY、Se+IL-2、Se+CY+IL-2)。硒源为富硒香茹粉末,掺入C57BL/6小鼠正常饲料,制成硒饲料,含硒约4ppm。非加硒饲料亦掺等量正常香茹粉末。各组小鼠先饲养4周,4周末各腹腔注射B16癌细胞1×10~3个。接受CY的3组,在接种B16后第3夭,腹腔注射CY注射液,剂量为50mg/kg,共一次;接受IL-2的3组,也在第3天始,连续腹腔注射IL-25天,上下午各2万单位,共20万单位。结果:单独3组与对照组经等级和非参数检验比较,只有IL-2组显示能延长荷瘤小鼠生存期(P<0.05),Se和CY单独无显著性,而Se+CY联合应用组有显著性(P<0.05);Se+IL-2联合组效果更显(P<0.01),但Se+CY+IL-2三者联合并未显示更高效果。提示硒和免疫制剂、抗癌剂联合应用效果优于单独应用。
Selenium (Se), cyclophosphamide (CY), and interleukin-2 (1L-2) alone and in combination were used to prolong the survival of tumor-bearing mice. The experiment was divided into 7 groups, 10 in each group: except for the control, 3 groups were used alone (Se, CY, IL-2), and 3 groups were combined (Se+CY, Se+IL-2, Se+CY+IL -2). The selenium source was selenium-enriched Xiangru powder, which was spiked into the normal feed of C57BL/6 mice and made into selenium feed, containing about 4 ppm of selenium. Non-selenium feed was also blended with normal Xiangru powder. Each group of mice was first fed for 4 weeks, and at the end of the 4 weeks, B16 cancer cells were intraperitoneally injected with 1×10~3 cells. The three groups receiving CY were injected with CY injection at a dose of 50 mg/kg 3 times after B16 inoculation. The three groups receiving IL-2 were also given intraperitoneal injection of IL-25 at the 3rd day. Days, 20,000 units in the previous and afternoon, totaling 200,000 units. RESULTS: Compared with the control and non-parametric tests in the three groups alone, only the IL-2 group could prolong the survival of the tumor-bearing mice (P<0.05). There was no significant difference between Se and CY alone, and Se+CY was used in combination. The group was significant (P<0.05); the combination of Se+IL-2 was more effective (P<0.01), but the combination of Se+CY+IL-2 did not show higher effect. It is suggested that the combined effect of selenium, immunological preparations and anticancer agents is superior to that of single application.