【目的】分析不同训练阶段血液生化指标的变化规律，研究调教训练方案的可行性，为青年伊犁马的调教训练提供数据参考。【方法】通过对8匹青年伊犁马制定坡道训练与间歇训练相结合的专项训练方案，进行为期6周的调教训练，分别于训练第0周、第2周、第4周、第6周检测血浆中谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷丙转氨酶/谷草转氨酶(ALT/AST)，肌酸激酶(CK)、乳酸脱氢酶(LDH)、肌酐(CREA)、甘油三酯(TG)、总蛋白(TP)、尿酸(UA)、乳酸(LAC)、钙离子(Ca2+)、磷离子(P+)12项血液生化指标，对其进行方差分析，并对比不同训练阶段的变化规律。【结果】赛前ALT和ALT/AST训练第4周显著高于训练前(P<0.05)，TG和Ca2+浓度训练第6周显著低于训练前(P<0.05)；赛后即刻ALT、ALT/AST和CREA训练第4周极显著高于训练前(P<0.01)，LAC浓度训练第6周显著高于训练前，Ca2+浓度训练第6周显著低于训练前(P<0.05)。【结论】调教训练方案对试验马匹产生了积极作用，其无氧代谢能力增强。调教训练方案可作为马匹调教训练的参考方案。 【Objective】 To analyze the changing rules of blood biochemical indexes in different training stages and to study the feasibility of adjusting training programs so as to provide reference for the training of young Ili horses. 【Method】 Eight-month-old Yili horse was trained on a combination of hill training and intermittent training to conduct training training for 6 weeks. The training training was carried out on weeks 0, 2, 4, 6 The levels of ALT, AST, ALT / AST, CK, LDH, CREA, triglyceride Twelve blood biochemical parameters of TG, TP, UA, LAC, Ca2 +  and P +  were measured and their variance Analyze, and compare the variation of different training phases. 【Results】 The results showed that the training ALT and ALT AST in the fourth week was significantly higher than that before training (P <0.05), while the concentrations of TG and Ca2 +  in the sixth week were significantly lower than those before training (P < lt; 0.05). The ALT, ALT, AST and CREA training scores in the fourth week after training were significantly higher than those before training (P <0.01), and the LAC training at 6 weeks was significantly higher than that before training Concentration training at 6 weeks was significantly lower than that before training (P <0.05). 【Conclusion】 Tuning training programs have a positive effect on test horses, and their anaerobic metabolism is enhanced. Tuning training program can be used as a reference training program for horse tuning. 