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采用培养的内皮素所致血管平滑肌细胞增殖模型。用氚-胸腺嘧啶核苷掺入法、流式细胞术、Westernblot及Northernblot分析方法，观察了莲心减对血管平滑肌细胞增殖的作用及对热应激蛋白70及其mRNA和抑癌基因P53表达的影响。结果发现，遂心碱能逆转内皮素所致的~3H－TdR掺入量增多，阻止血管平滑肌细胞由Go／G1期进入DNA合成期（S期）和有丝分裂期（G2／M期）．并能使效应激蛋白70及mRNA表达减弱，P53抑癌基因及mRNA表达增强。以上结果提示，莲心碱能抑制血管平滑肌细胞增殖，这可能与热应激蛋白70及P53的调控有关。
Vascular smooth muscle cell proliferation model induced by cultured endothelin. The effect of Lianxinxiao on the proliferation of vascular smooth muscle cells and the expression of heat stress protein 70 and its mRNA and tumor suppressor gene P53 were investigated by indole-thymidine incorporation, flow cytometry, Western blot and Northern blot analysis. influences. The results showed that scopolamine could reverse the endothelin-induced increase in ~3H-TdR incorporation and prevent vascular smooth muscle cells from Go/G1 phase into the DNA synthesis phase (S phase) and mitosis phase (G2/M phase). And can reduce the expression of effector 70 and mRNA, P53 tumor suppressor gene and mRNA expression. The above results suggest that neomycin can inhibit the proliferation of vascular smooth muscle cells, which may be related to the regulation of heat stress protein 70 and P53.