论文部分内容阅读
目的 分析非小细胞肺癌 (NSCLC)淋巴结转移与血管生成及p16、p5 3蛋白表达的关系。方法 采用免疫组织化学方法对 187例NSCLC(74例腺癌、76例鳞癌和 37例腺鳞混合癌 )进行血管染色计数和p16、p5 3蛋白测定 ,并作相关临床因素分析。结果 非小细胞肺癌组织中微血管密度平均为 6 .8± 3.3,p16和p5 3蛋白阳性表达率分别为 4 7.6 %和 34.2 %。各组织类型非小细胞肺癌中淋巴结转移病例 (T2 N1～ 2 M0 )的微血管密度显著高于未转移者 (T2 N0 M0 ) ;在肺鳞癌和腺鳞癌中 ,p16和p5 3蛋白同时表达异常 (p16 -p5 3+ )与微血管密度增高有关 ,但与淋巴结转移无关 ;而在肺腺癌中 ,p16和p5 3蛋白同时表达异常与淋巴结转移有关 ,但与微血管密度无关。微血管密度和p16、p5 3蛋白表达异常与年龄、性别、病理类型及分化程度均无明显相关。结论 各组织类型非小细胞肺癌的淋巴结转移均与活跃的血管生成密切相关 ,其中在肺鳞癌和腺鳞癌中 ,血管生成与p16和p5 3基因表达异常有关。而在肺腺癌中 ,血管生成和p16、p5 3蛋白异常表达是影响其淋巴结转移的两项独立的因素。
Objective To analyze the relationship between lymph node metastasis and angiogenesis and p16, p5 protein expression in non-small cell lung cancer (NSCLC). Methods 187 cases of NSCLC (74 cases of adenocarcinoma, 76 cases of squamous cell carcinoma and 37 cases of squamous cell carcinoma of the squamous cell carcinoma) were subjected to immunohistochemical staining for blood vessel counting and determination of p16 and p5 proteins, and related clinical factors were analyzed. Results The average microvessel density of non-small cell lung cancer was 6.8 ± 3.3. The positive rates of p16 and p5 3 protein were respectively 4. 7.6% and 34.2%. The microvessel density of T2 N1 ~ 2 M0 in patients with non-small cell lung cancer was significantly higher than that in non-metastasis (T2 N0 M0). In both squamous cell carcinoma and adenosquamous carcinoma, the expression of p16 and p5 3 protein Abnormalities (p16-p5 3+) are associated with increased microvessel density but are not associated with lymph node metastasis; whereas in lung adenocarcinomas, simultaneous expression of p16 and p53 proteins is associated with lymph node metastasis, but not with microvessel density. Microvessel density and p16, p5 protein expression abnormalities and age, gender, pathological type and degree of differentiation were not significantly correlated. Conclusions Lymph node metastasis in each tissue type non-small cell lung cancer is closely related to active angiogenesis. Among them, angiogenesis is related to the abnormal expression of p16 and p53 genes in squamous cell carcinoma and adenosquamous carcinoma. In lung adenocarcinoma, abnormal expression of angiogenesis and p16, p5 3 proteins are two independent factors affecting their lymph node metastasis.