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目的 :观察急性缺氧时血浆、组织内源性一氧化碳的变化及外源性一氧化碳对急性肺动脉高压的影响。方法 :实验分为两部分 :实验 1 :30只雄性Wistar大鼠随机分为 3组 :组I (对照组 ) :大鼠吸入 2 1 %O23 0min ;组II(缺氧组 ) :大鼠吸入 1 0 %O2 3 0min ;组III (ZnPPIX组 ) :腹腔注入血红素氧化酶抑制剂ZnPPIX 1 0μmol/kg后 ,大鼠吸入 1 0 %O2 3 0min。实验结束时取血样进行一氧化碳及环磷酸鸟苷分析 ,取组织样品进行匀浆、离心测组织一氧化碳含量 ;实验 2 :1 0只大鼠吸入 1 0 %O2 2 0min ,待肺动脉压力曲线恢复基线后 ,给大鼠吸入50× 1 0 - 6 的一氧化碳与缺氧气体的混合气 30min ,观察血流动力学和血气的变化。结果 :急性缺氧增加了血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量 ,ZnPPIX降低了缺氧大鼠的血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量 ,缺氧明显升高了平均肺动脉压 ,而吸入 50× 1 0 - 6 的一氧化碳可明显降低肺动脉压 ,而且吸入一氧化碳可明显增加缺氧大鼠的动脉氧分压。结论 :急性缺氧明显增加了血浆、组织一氧化碳含量及血浆环磷酸鸟苷含量。外源性一氧化碳可明显降低缺氧时肺动脉高压
Objective: To observe the changes of plasma and tissue endogenous carbon monoxide during acute hypoxia and the effect of exogenous carbon monoxide on acute pulmonary hypertension. Methods: The experiment was divided into two parts: Experiment 1: 30 male Wistar rats were randomly divided into 3 groups: Group I (control group): rats were infused with 21% O23 0min; Group II (hypoxia group) 10% O2 3 0min; Group III (ZnPPIX): Rats were inhaled 10% O2 3 0min after intraperitoneal injection of heme oxygenase inhibitor ZnPPIX 10μmol / kg. At the end of the experiment, blood samples were taken for analysis of carbon monoxide and guanosine monophosphate. Tissue samples were taken for homogenization and centrifuged to measure the content of carbon monoxide. Experiment 2: 10 rats were ingested with 10% O2 2 0 min. After the pulmonary pressure curve recovered to baseline The rats were inhaled 50 × 10-6 carbon monoxide and oxygen-deficient gas mixture 30min, hemodynamics and blood gas changes were observed. Results: Acute hypoxia increased plasma and tissue carbon monoxide levels and plasma cyclic guanosine monophosphate content. ZnPPIX decreased plasma and tissue carbon monoxide levels and plasma cyclic guanosine monophosphate levels in hypoxic rats. Hypoxia significantly increased mean pulmonary arterial pressure , While inhalation of 50 × 10 -6 carbon monoxide can significantly reduce pulmonary arterial pressure, and carbon monoxide inhalation can significantly increase the arterial oxygen pressure in hypoxic rats. Conclusion: Acute hypoxia significantly increased plasma and tissue carbon monoxide levels and plasma cyclic guanosine monophosphate content. Exogenous carbon monoxide can significantly reduce pulmonary hypoxia hypoxia