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采用微透析技术获得杨树质外体透析液,高效液相色谱测定盐胁迫下吴屯杨和欧美杨内源激素的动态变化,并结合叶绿素荧光参数的变化,分析对比吴屯杨和欧美杨两种杨树的耐盐性。结果表明,高盐度胁迫下吴屯杨体内脱落酸(ABA)的积累量为对照的1.3倍,而欧美杨体内ABA的积累量为对照的1.8倍;欧美杨的PSII原初光能转换效率(F v/F m)及电子传递效率(ETR)值显著低于吴屯杨,光系统破坏程度较大;欧美杨的抗盐平衡系数明显大于吴屯杨的抗盐平衡系数,说明盐胁迫下欧美杨各种激素之间的协调作用下降,生长受胁迫的抑制程度大于吴屯杨,表明吴屯杨耐盐性强于欧美杨。此方法为研究杨树质外体内的耐盐性机制和选育耐盐杨树新品种提供理论依据。
Populus apoplastic dialysis liquid was obtained by microdialysis technique and the dynamic changes of endogenous hormones in Populus euphratica and Populus euphratica under salt stress were measured by HPLC. The changes of chlorophyll fluorescence parameters were compared with those of Wu Tun Yang and Ouyang Yang Salt tolerance of poplar. The results showed that under high salinity stress, the accumulation of abscisic acid (ABA) was 1.3 times higher than that of the control, while the accumulation of ABA in Populus euchemiticus was 1.8 times higher than that of the control. The initial photosynthetic efficiency of PSII F v / F m) and electron transfer efficiency (ETR) were significantly lower than that of Wu Tun Yang, and the damage of light system was greater. The salt-resistant balance coefficient of Populus euphratica was significantly higher than that of Wu Tun Yang, indicating that under salt stress The coordination between the various hormones in Europe and the United States decreased, and the inhibition of growth was greater than that of Wu Tun Yang, indicating that the salt tolerance of Wu Tun Yang was stronger than that of Europe and the United States. This method provides a theoretical basis for studying the mechanism of salt tolerance in poplar extracellular body and breeding new varieties of salt-resistant poplar.