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本文研究的是一个相互易位t(2;5)(p11;q15)男性携带者的精子染色体。精子染色体的取材来自受精后的仓鼠卵的游离带区。方法见Kartin(1983)技术。共完成了四次实验,其中三次实验用的是新鲜的精液;一次用的是在4℃卵黄缓冲液中存放了2天的精液,且用量是第三次实验所用的一半。染色体分别作了Q显带、G显带以及常规Giemsa染色。总共分析了75个中期染色体,其中59个(78.6%)的精子染色体组分是由于2∶2分离所致,16个(21.3%)是由于3∶1分离所致,4∶0分离类型未见到。而在26个2∶2分离显带质量好的中期分裂相中,有15个(20%)的核型为正常或平衡易位,7个(9.3%)为邻位1分离,
This article investigates the sperm chromosome of a male carrier with a reciprocal translocation t (2; 5) (p11; q15). Sperm chromosomes were drawn from the free zone of hamster eggs after fertilization. Methods see Kartin (1983) technique. A total of four experiments were performed, of which three used fresh semen; one used semen stored for two days at 4 ° C in yolk buffer and used in half the amount used for the third experiment. Chromosomes were made Q banding, G banding and conventional Giemsa staining. A total of 75 metaphase chromosomes were analyzed, of which 59 (78.6%) were due to 2: 2 segregation, 16 (21.3%) due to 3: 1 segregation, 4:0 segregation type See. In 26 2: 2 bands with good banding quality, 15 (20%) showed normal or equilibrium translocations and 7 (9.3%) showed ortho-1 separation,