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在选择观察的６例流行性出血热（ＥＨＦ）患者中（重型２例，中型２例，轻型２例），发现有２例重型和１例中型患者的外周血单个核细胞（ＰＢＭＣ）表达肿瘤坏死因子α（ＴＮＦα）ＴｎＲＮＡ，特别以低血压休克期表达最为明显。同时应用免疫ＰＡＰ法对ＰＢＭＣ进行染色显示，病情重伴低血压休克或有明显肾功能损害的患者ＰＢＭＣ中ＴＮＦα阳性细胞数增加。ＥＨＦ患者除单核／巨噬细胞外，部分淋巴细胞和肾小管细胞ＴＮＦα染色亦呈阳性。动态观察ＥＨＦ患者（１０例重型、１０例中型、１０例轻型，其中包含前述６例患者）血浆ＴＮＦα变化发现，血浆ＴＮＦα的水平与临床症状程度成正比，重型病例的血浆ＴＮＦα水平要高于轻型的（发热期重型ｎ＝７，３１．６３±１２．３６ｐｍｏｌ／Ｌ，ｖｓ轻型ｎ＝１０，１９．８９±５．９４ｐｍｏｌ／Ｌ，Ｐ＜０．０５），同一病例发病的不同时期，以低血压休克期升高最为明显。血浆ＴＮＦα水平与肾小球滤过功能损伤、肾近端和远端小管损伤程度密切相关。揭示ＴＶＦα可能是造成ＥＨＦ发病，特别是低血压休克和肾损害的重要介质。应用单抗等拮抗ＴＮＦα，有可能成为今后ＥＨＦ临床治疗中一个重要的措施。
Of the 6 patients with selected epidemic hemorrhagic fever (EHF) who were selected for observation (2 with severe, 2 with medium and 2 with light), peripheral blood mononuclear cells (PBMCs) were found to express tumors in 2 heavy and 1 medium-sized patients Necrosis factor alpha (TNFα) TnRNA, especially in hypotensive shock expression of the most obvious. At the same time, immunohistochemical PAP staining of PBMC showed that the number of TNFα-positive cells in PBMC of patients with severe concomitant hypotension or impaired renal function increased. EHF patients in addition to monocytes / macrophages, some lymphocytes and tubular cells TNFα staining was also positive. Dynamic observation of EHF patients (10 cases of heavy, 10 cases of medium and 10 cases of light, including 6 cases of patients with plasma TNFα changes found that the level of plasma TNFα is proportional to the degree of clinical symptoms, severe cases of plasma TNFα levels higher than the light (N = 7, 31.63 ± 12.36pmol / L, n = 10, 19.89 ± 5.94pmol / L, P <0.05) in different stages of the same case Hypoblasts increased the most obvious shock. Plasma TNFα levels and glomerular filtration injury, renal proximal and distal tubule damage is closely related. Revealing that TVFα may be an important mediator in the pathogenesis of EHF, especially in hypotensive shock and renal damage. The use of monoclonal antibody and other antagonistic TNFα, may become an important measure in the future clinical treatment of EHF.