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[目的]研究铽纳米颗粒的制备新方法及其荧光性质、颗粒表征及BSA对其荧光强度的影响。[方法]以单宁酸为还原剂,利用在表面活性剂十二烷基苯磺酸钠(SDBS)形成的微乳环境中将硝酸铽还原成水溶性铽(Tb)纳米颗粒;利用紫外分光光度计、荧光分光光度计、透射电子显微镜等研究铽纳米颗粒的性质,并通过修饰剂巯基丙酸与BSA连接,分析铽纳米颗粒与BSA连接前后的荧光变化。[结果]透射电子显微镜显示随着还原剂加入量的增加,形成的球形铽纳米颗粒直径逐渐减小;同时荧光光谱显示,纳米颗粒的直径从12 nm到20 nm,以275 nm波长激发,发射峰位置分别为393.8、388.8、387.2、384.0、384.0 nm,逐渐下移。铽纳米颗粒与BSA结合后荧光强度明显增强。[结论]提供了一种新的制备稀土元素铽纳米颗粒的方法,利用该方法可以制备大小不同的铽纳米颗粒,并且与蛋白结合后荧光强度明显增强,在生物大分子检测等领域具有广泛的应用前景。
[Objective] The research aimed to study a new preparation method of Tb nanoparticles and their fluorescent properties, particle characterization and the influence of BSA on their fluorescence intensity. [Method] With tannic acid as reductant, terbium nitrate was reduced to water-soluble terbium (Tb) nanoparticles in the microemulsion environment formed by the surfactant sodium dodecylbenzenesulfonate (SDBS). Ultraviolet spectroscopy The properties of terbium nanoparticles were studied by photometer, fluorescence spectrophotometer and transmission electron microscope. The fluorescence of terbium nanoparticles before and after the attachment of BSA to BSA was analyzed by the conjugation of mercaptopropionic acid with BSA. [Result] Transmission electron microscopy showed that the diameter of spherical terbium nanoparticles decreased with the addition of reducing agent. The fluorescence spectra showed that the diameters of nanoparticles ranged from 12 nm to 20 nm and were excited at 275 nm and emitted Peak positions were 393.8,388.8,387.2,384.0,384.0 nm, gradually down. The fluorescence intensity of terbium nanoparticle combined with BSA increased significantly. [Conclusion] A new method for preparing rare earth terbium nanoparticles was provided. Terbium nanoparticles with different sizes could be prepared by this method. The fluorescence intensity of the Tb nanoparticles increased obviously after binding with the protein and had a wide range of applications in the fields of biological macromolecule detection Application prospects.