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采用均匀设计法对衍生化 β-环糊精 (β- CD)键合固定相的合成条件进行优化 ,考察的主要因素包括衍生化试剂异氰酸苯酯的用量、反应时间及反应温度。衍生化后β- CD键合相的碳含量优化结果预测值和实测值分别为17.95 %和 18.10 %。采用红外光谱、热重分析曲线对衍生化前后β- CD键合固定相进行表征 ,探讨了衍生化前后β-CD键合相的手性拆分能力。
The synthesis conditions of β-cyclodextrin (β-CD) bonded stationary phase were optimized by uniform design. The main factors involved were the amount of phenylisocyanate, the reaction time and the reaction temperature. The predicted and measured carbon content of derivatized β-CD bonded phase was 17.95% and 18.10%, respectively. The β-CD bonded stationary phases before and after derivatisation were characterized by infrared spectroscopy and thermogravimetric analysis, and the chiral resolution ability of β-CD bonded phase before and after derivatisation was discussed.