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利用自旋标记电子自旋共振（ＥＳＲ）技术，测定急性运动中及不同时程恢复期血浆抗坏血酸自由基（ＡＦＲ）和脂质过氧化水平（ＭＤＡ）的改变，同时观察红细胞膜（ＲＢＣＭ）脂流动性的变化。结果表明：血浆ＡＦＲ信号强度和ＭＤＡ水平在力竭运动后即刻均显著升高（均Ｐ＜０．００５），运动后３０ｍｉｎ仍维持高水平（均Ｐ＜０．０５），随后二者逐渐恢复，呈平行变化。ＲＢＣＭ脂尾部流动性在短时间中等强度运动（三级负荷末）即有明显下降（Ｐ＜０．０５），运动后恢复期进行性加重，并于运动后１２小时达非常显著改变（Ｐ＜０．０１）；此时膜脂头部流动性也明显下降（Ｐ＜０．０５），之后二者均有恢复趋势；提示递增负荷力竭性运动对ＲＢＣＭ脂流动性有重要影响，并且在运动诱导的氧化应激中，ＡＦＲ可作为一个有效的抗氧化指标，同时也反映了运动过程中及恢复期机体氧化、抗氧化系统的动态平衡。
The changes of plasma ascorbic acid radical (AFR) and lipid peroxidation (MDA) during acute exercise and at different recovery stages were measured by spin-labeled ESR technique. Meanwhile, the erythrocyte membrane (RBCM) lipid Liquidity changes. The results showed that plasma AFR signal intensity and MDA level increased significantly immediately after exhaustive exercise (all P <0.005), remained high at 30 min after exercise (both P <0.05), and then gradually recovered , Was parallel changes. RBCM lipid tail mobility in the short-term moderate-intensity exercise (three load end) that is significantly decreased (P <0.05), post-exercise convalescent progressive aggravation, and 12 hours after exercise significantly up (P < 0.01). At the same time, the fluidity of membrane lipids decreased significantly (P <0.05), and then both showed a tendency of recovery. It was suggested that progressive exhaustive exercise exerted an important influence on the fluidity of RBCM, In exercise-induced oxidative stress, AFR can be used as an effective antioxidant index, and also reflects the dynamic balance of the body’s oxidation and anti-oxidant system during and after exercise.