论文部分内容阅读
目的:探讨CD38/138/45/SSC及CD38/56/19/45/SSC四色组合流式细胞术在多发性骨髓瘤(MM)患者诊断中的临床应用.方法:采用多参数流式细胞技术,通过CD38/CD45双抗体设门,以CD38/138/45/SSC及CD38/56/19/45/SSC四色组合检测33例确诊MM患者骨髓中的浆细胞,并进行浆细胞胞浆κ/λ的检测.结果:33例MM患者中均可检测到CD38++/CD138+/CD45+/-细胞群,在该细胞群中并未找到56/19+的正常浆细胞,免疫表型为45-/56+/19-标本26例,占79％,45 /56-/19标本5例,占15％,45+/56+/19标本2例,占6％.对上述CD38++/138+标本进行胞浆κ/λ检测,均为单克隆瘤细胞.结论:CD38/138/45/SSC及CD38/56/19/45/SSC四色流式细胞检测能精确发现恶性变的浆细胞,在MM诊断中有重要的临床意义和诊断价值.“,”Objective:To explore the diagnosis value of CD38/138/45/SSC and CD38/56/19/45/SSC four colour flow cytometry panel in multiple myeloma (MM).Method:We used the CD38/CD45 double antibody setting gate with CD38/138/45/SSC and CD38/56/19/45/SSC four colour panel to analyze bone marrow cytoplasm of 33 cases with MM,and detected cytoplasm κ/λ,in all cases.Result:All of 33 cases could be found out the CD38++/CD138+/CD45+/ cells.The normal plasma cells of CD56-/CD19+ cannot be found in the group.There were 26 cases (79％) of 45 /56+/19,5 cases (15％) of 45-/56 /19-and 2 cases (6％) of 45+/56+/19-.All the cases with cytoplasm κ/λ,detection were confirmed monoclonal tumor cells.Conclusion:The use of CD38/138/45/SSC and CD38/56/19/45/SSC four colour flow cytometry panel can accurately distinguish myeloma cells.It\'s very helpful for the diagnosis of MM.