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目的建立了一种基于Fe~(3+)催化的荧光增强的传感平台用于抗坏血酸的灵敏检测。方法抗坏血酸可以和邻苯二胺反应生成一种具有π键共轭结构的氮杂环的荧光化合物,从而实现检测抗坏血酸;Fe~(2+)能够加速上述反应的进程。结果在0.2~1.7 mmol/L浓度范围内,体系荧光信号和抗坏血酸浓度成线性关系,相关系数r=0.997,检测限为0.35μmol/L。此外,常见的干扰物不影响抗坏血酸的选择性测定。结论该方法具有较高的灵敏度,低廉的成本和简便的操作步骤,并且易于实现定量分析。
OBJECTIVE To establish a fluorescence-enhanced sensing platform based on Fe ~ (3+) catalysis for the sensitive detection of ascorbic acid. Methods Ascorbic acid can react with o-phenylenediamine to form a fluorescent compound with π-conjugated nitrogen heterocycle to realize the detection of ascorbic acid. Fe 2+ can speed up the reaction. Results The linear relationship between the fluorescence signal and the concentration of ascorbic acid was found in the concentration range of 0.2-1.7 mmol / L, with a correlation coefficient of 0.997 and a detection limit of 0.35 μmol / L. In addition, common interferences do not affect the selectivity of ascorbic acid. Conclusion The method has high sensitivity, low cost and easy operation steps, and easy to achieve quantitative analysis.