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目的:探讨司坦唑醇对糖皮质激素(GC)所致大鼠骨质疏松的骨密度和力学性能影响。方法28只3月龄雄性SD大鼠,体质量(226±12)g,随机等分为基础对照(A)组、年龄对照(B)组、激素模型(C)组和司坦唑醇预防(D)组。C组和D组大鼠均喂醋酸泼尼松4.5 mg/kg·b.w.,每周2次,D组同时每天饲喂司坦唑醇0.5 mg/kg·b.w.。3月后,用双能X线吸收骨密度测量扫描仪对各组的离体双侧股骨和第5腰椎进行骨密度测定,然后用SWD-10型材料试验机行股骨干的扭转、三点弯曲和腰椎的压缩试验。结果与B组比较,C组股骨、腰椎总骨密度减少了14.64%(P<0.01);右、左股骨远段和腰椎的骨密度分别减少了21.42%(P<0.01)、19.62%(P<0.05)和23.48%(P<0.01);与C组比较,D组的BMD 均稍有增加。与B组比较,C组股骨三点弯曲的载荷减少了17.1%(P<0.05),其余的力学参数都有减少的趋势;与C组比较,司坦唑醇预防组股骨扭转角度增加了72.5%(P<0.05),其余的力学参数也都有增加的趋势。结论长期使用GC,会使大鼠股骨、腰椎的骨密度和力学性能下降;司坦唑醇则能防止GC所致骨量丢失,还能增加其力学性能。
Objective: To investigate the effect of stanozolol on bone mineral density and mechanical properties of rats with osteoporosis induced by glucocorticoid (GC). Methods 28 male SD rats of 3 months old were randomly divided into control group (A), age group (B), hormone group (C) and stanozolol (D) group. Rats in group C and group D were fed with 4.5 mg / kg b.w. prednisone acetate twice a week, while group D was fed with 0.5 mg / kg b.w. Three months later, with double energy X-ray absorptiometry measurement of bone density scanner bilateral pairs of femur in each group and the fifth lumbar spine bone mineral density was measured, and then SWD-10 material testing machine torsion of the femoral shaft, three Bending and lumbar compression test. Results Compared with group B, the total BMD of femur and lumbar in group C decreased by 14.64% (P <0.01). The BMD of distal and lumbar femur of group C decreased by 21.42% (P <0.01) and 19.62% (P <0.05) and 23.48% (P <0.01). Compared with group C, the BMD in group D increased slightly. Compared with group B, the load of three-point bending of femur in group C decreased by 17.1% (P <0.05), and the rest of the mechanical parameters tended to decrease. Compared with group C, the angle of femoral torsion increased by 72.5 % (P <0.05), the rest of the mechanical parameters also have an increasing trend. Conclusion Long-term use of GC can reduce the bone mineral density and mechanical properties of femur and lumbar in rats. Stanozolol can prevent the loss of bone mass caused by GC and increase its mechanical properties.