由于缺少评价口服菌苗免疫原性的方法,必须通过大规模现场试验来检验每种新候选菌苗的效力与合适免疫剂量。本文报道一种通过计数免疫后血清中抗体分泌细胞数目来测定口服菌苗的免疫原性的方法。其原理为:口服免疫后,小肠淋巴组织中的淋巴细胞被致敏迁移到局部淋巴结成熟,然后通过血循环回到肠粘膜。此淋巴细胞可在外周血中存在,其合成的抗体可在体外测定。作者应用此法测定了口服伤寒活菌苗Ty21a的局部免疫反应情况。首先,将80个志愿者分 Due to the lack of methods for assessing the immunogenicity of oral vaccines, the efficacy and appropriate immunization dose of each new vaccine candidate must be examined through large-scale field trials. Here we report a method for determining the immunogenicity of oral vaccines by counting the number of antibody-secreting cells in the serum after immunization. The principle is: After oral immunization, lymphocytes in the small intestine lymphoid tissue are sensitized and migrate to mature local lymph nodes and then return to the intestinal mucosa through the blood circulation. This lymphocyte can exist in peripheral blood and its synthetic antibody can be measured in vitro. The authors applied this method to determine the local immune response of Ty21a oral vaccine. First, divide 80 volunteers