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口服脊髓灰质炎疫苗 ( OPV)是全球消灭脊髓灰质炎的主要手段。有经验显示Sabin疫苗病毒在 Vero细胞中培养的毒力回复突变比在原代猴肾细胞中培养的高。本研究以聚合酶链反应和限制性内切酶酶切技术作突变分析 ( MAPREC) ,分析经 Vero细胞或次代绿猴肾 ( s GMK)细胞培养的脊髓灰
Oral polio vaccine (OPV) is the world’s primary means of eradicating poliomyelitis. Experience has shown that the virulence-back mutation in the Sabin vaccine virus cultured in Vero cells is higher than that cultured in primary monkey kidney cells. In this study, polymerase chain reaction and restriction enzyme digestion technology for mutation analysis (MAPREC), analysis of Vero cells or second-generation green monkey kidney (s GMK) cell culture of spinal gray