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目的观察食物过敏症婴幼儿血清中IL-10和转化生长因子-β(TGF-β)的变化,探讨其在婴幼儿食物过敏症致病中的作用。方法选择食物过敏症婴幼儿40例(食物过敏组),另选取年龄、性别相匹配的健康婴幼儿40例作为健康对照组,通过Uni-CAP100E系统检测食物过敏组及健康对照组婴幼儿血清食物特异性IgE(SIgE),采用ELISA法检测2组婴幼儿血清IL-10和TGF-β水平。结果食物过敏组婴幼儿均有皮肤、呼吸道和(或)消化道表现,血清食物SIgE检测有一种或多种食物SIgE阳性。其中牛奶过敏34例,鸡蛋过敏27例,且SIgE定量分析均在2级以上。健康对照组婴幼儿血清食物SIgE阴性,且平时无食物过敏的临床表现。食物过敏组血清IL-10水平[(10.28±2.38)ng.L-1]较健康对照组[(4.75±0.92)ng.L-1]显著升高(P=0.035)。食物过敏组TGF-β水平[(1 041.93±75.64)ng.L-1]显著高于健康对照组[(734.29±39.67)ng.L-1](P=0.001)。结论食物过敏是由免疫机制介导的过敏性疾病,细胞因子IL-10和TGF-β可能参与了婴幼儿食物过敏的发生,并可能在其中发挥重要作用。
Objective To observe the changes of serum IL-10 and transforming growth factor-β (TGF-β) in children with food allergy and to explore its role in the pathogenesis of food allergy in infants. Methods Forty infants and young children with food allergy (food allergy group) were enrolled in this study. Forty healthy infants with age and gender matched were selected as healthy control group. Uni-CAP100E was used to detect serum food in children with food allergy and healthy controls Specific IgE (SIgE) was measured by ELISA. Serum levels of IL-10 and TGF-β were measured in two groups of infants. Results Food allergy group infants and young children have skin, respiratory and (or) digestive tract performance, serum food SIgE detected one or more foods SIgE positive. Among them, 34 cases were milk allergy and 27 cases were egg allergy. The quantitative analysis of SIgE was above level 2. Serum food SIgE of healthy control group was negative, and usually no clinical manifestations of food allergy. The level of IL-10 in food allergy group was significantly higher than that in healthy control group [(10.28 ± 2.38) ng.L-1] (4.75 ± 0.92 ng.L-1) (P = 0.035). The level of TGF-β in food allergy group [(1041.93 ± 75.64) ng.L-1] was significantly higher than that in healthy controls [(734.29 ± 39.67) ng.L-1] (P = 0.001). Conclusions Food allergy is an allergic disease mediated by immune system. IL-10 and TGF-β may play an important role in food allergy in infants and young children.