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目的:观察比较3种酶联免疫吸附试验试剂检测丙型肝炎抗体的结果。方法:选取我中心门诊部2010年11月-2016年11月期间检测丙型肝炎筛选的血液样本100例进行观察,对其进行3种酶联免疫吸附试验试剂检查,分别为A试剂,(A组),B试剂(B组)以及C试剂(C组)。A组、B组均采用间接法进行检测,C组采用夹心法进行检测。对比3组血液样本的阳性检出情况。结果:C组使用夹心法真阳性标本检出率为95.24%,明显优于A组(44.44%)、B组(34.48%),P<0.05。结论:夹心法酶联免疫吸附试验试剂在丙型肝炎抗体的检测中的效果更佳,优于间接法。
Objective: To observe and compare the results of three kinds of enzyme-linked immunosorbent assay reagents for detecting hepatitis C antibody. Methods: 100 blood samples from hepatitis C screening in our Central Clinic from November 2010 to November 2016 were selected for observation. Three kinds of enzyme-linked immunosorbent assay reagents (A reagent, A Group), B reagent (group B), and C reagent (group C). Group A and group B were detected by indirect method and group C by sandwich method. Comparison of 3 groups of blood samples were positive detection. Results: The detection rate of true positive samples in group C was 95.24%, which was significantly higher than that in group A (44.44%) and group B (34.48%), P <0.05. Conclusion: Sandwich ELISA reagents are more effective in the detection of hepatitis C antibody than the indirect method.