本文报道从组织标本中鉴定T、B淋巴细胞的一种方法。该方法用超声震荡技术获得活的、相对纯的淋巴细胞,再利用它们的表面标志来分类。这一容易重复的方法可以作为一种标准的实验室方法,用以判定需要区分淋巴细胞来源的疾病状态。方法如下: 来自淋巴结、脾或胸腺的新鲜活检组织标本(约5mm~3)置培养皿中用解剖刀切碎,移入含10ml组织培养液(Mc Coy’s 5A)的烧杯中,于4℃冰水上经超声清洗器(型号ME 1.5,Mettler Elect- This article reports a method for identifying T, B lymphocytes from tissue specimens. The method uses ultrasound technology to obtain live, relatively pure lymphocytes and classify them using their surface markers. This easy-to-repeat method can be used as a standard laboratory method to determine the need to differentiate lymphocyte-derived disease states. Methods: Fresh biopsies from lymph nodes, spleen or thymus (approximately 5 to 3) were placed in petri dishes and cut into scallops and transferred to beakers containing 10 ml tissue culture (Mc Coy’s 5A) on ice water at 4 ° C The ultrasonic cleaner (model ME 1.5, Mettler Elect-