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本文介绍一种简便易行并可在光、电镜下研究单个神经元形态和神经元回路的方法。此法主要包括荧光逆行标记、固定脑片细胞内电泳ＬｕｃｉｆｅｒＹｅｌｌｏｗ和ＤＡＢ光转化三个步骤。它主要具有以下优点；（１）利用送行追踪确定神经元投射部位；（２）进一步细胞内注射可充分显示神经元的树突构筑，尤其是远端树突和树突棘；（３）经ＤＡＢ转化的神经元形象能长久保存，克服了荧光易褪色的缺点，可供详细观察和分析；（４）转化后的细胞，因已经过良好固定，结构保存较好；故可在电镜下进一步观察研究；（５）结合免疫荧光，通过Ｃｏｎｆｏｃａｌ显微镜可更清晰地观察ＬｕｃｉｆｅｒＹｅｌｌｏｗ标记的细胞与其它神经纤维的联系；（６）此外还可用于病理检查的标本，研究人体脑组织神经元的形态特征。为此，此方法不失为一种研究单个神经元形态（尤其是树突构筑）和神经元回路的良好方法。
This article describes a simple and easy and can be studied under light and electron microscopy single neuron morphology and neuronal circuits. This method includes fluorescent retrograde labeling, fixed intracell electrophoresis of Lucifer Yellow and DAB light conversion in three steps. It mainly has the following advantages: (1) using transmission tracking to determine neuronal projection site; (2) further intracellular injection can fully display dendritic structure of neurons, especially distal dendrites and dendritic spines; (3) DAB transformed neurons can be long-term preservation, to overcome the shortcomings of fluorescent easy to fade, for detailed observation and analysis; (4) transformed cells, as has been well fixed, the structure is better preserved, it can be further under electron microscopy (5) In combination with immunofluorescence, LuciferYellow labeled cells can be observed more clearly by Confocal microscope; (6) In addition, it can be used for pathological examination of the morphological characteristics of human brain tissue neurons . For this reason, this method is a good way to study single neuron morphology (especially dendritic construction) and neuronal circuits.