体外培养人滑膜细胞是研究人类关节炎症性疾患的重要手段。人滑膜细胞培养一般需要在关节手术或关节镜活检时设法获取滑膜组织,取材不易,而且滑膜细胞往往取自病情较重的患者,来源受限,致使这方面的研究工作存在重重困难。 本文报告了一种直接由关节液收集、培养滑膜细胞的新方法,其主要步骤包括:(1)收集关节液细胞:无菌取关节液30ml左右,1000r/min离心10min,弃上清,之后用Hanks液洗细胞3次。加入适量含10％胎牛血清的RPMI 1640培养液进行培养。(2)除去血液细胞成分:如上收集的细胞混有大量血细 Human synovial cells cultured in vitro is an important means to study the inflammatory diseases in human joints. Human synovial cell culture generally requires synovial tissue to be harvested during arthroscopic or arthroscopic biopsies. Difficult to obtain materials, synovial cells are often derived from patients with severe conditions and have limited sources of experience, making the study difficult in this area . This article reports a new method of collecting and culturing synovial cells directly from synovial fluid. The main steps include: (1) collecting synovial cells: about 30 ml of aseptic synovial fluid, centrifuging at 1000 r / min for 10 minutes, discarding the supernatant, Afterwards wash the cells 3 times with Hanks solution. Add appropriate amount of RPMI 1640 medium containing 10% fetal bovine serum for culture. (2) remove the blood cell components: the above collected cells mixed with a large number of blood