论文部分内容阅读
目的对照检测伴有正常及高级别上皮内瘤变的胃腺癌组织中定位于染色体9p21区D9S166、D9S171、D9S941、D9S942和IFNA的微卫星不稳定性(MSI),探讨p14、p15和p16基因与胃癌发生发展的关系。方法选择55例伴有正常及高级别上皮内瘤变的胃腺癌标本,应用手工显微切割、聚合酶链反应、高压凝胶电泳、硝酸银染色等技术,对照分析正常、高级别上皮内瘤变和胃腺癌组织中9p21上的5个微卫星位点的MSI变化。结果胃癌组织MSI总发生率为27%(64/233),高级别上皮内瘤变MSI总发生率为18%(42/233),正常组织中未发生MSI。胃癌组织中的MSI发生率显著高于高级别上皮内瘤变(P<0.05)。在胃癌组织中,5个微卫星位点的MSI发生率依次为D9S171(45%)、IFNA (41%)、D9S941(22%)、D9S166(16%)、D9S942(15%);高级别上皮内瘤变组织中,依次为IFNA (22%)、D9S171(21%)、D9S941(18%)、D9S166(14%)、D9S942(11%)。在胃癌组织中D9S171位点的MSI发生率为45%,在高级别上皮内瘤变的MSI发生率为21%。两者差异有统计学意义(P<0.05)。结论胃癌中染色体9p21区发生有高频率的MSI,MSI可能为胃癌发生过程中的早期分子事件,并且在胃癌发展过程中持续发挥作用。p14、p15和p16基因可能与胃癌的发生发展相关。
Objective To compare the microsatellite instability (MSI) of D9S166, D9S171, D9S941, D9S942 and IFNA located in chromosome 9p21 region in gastric adenocarcinoma with normal and high-grade intraepithelial neoplasia, and to explore whether p14, p15 and p16 genes and Relationship between gastric cancer and development. Methods Fifty-five specimens of gastric adenocarcinoma with normal and high-grade intraepithelial neoplasia were selected and analyzed by hand microdissection, polymerase chain reaction, high pressure gel electrophoresis and silver nitrate staining. Changes of MSI in 5 microsatellite loci on 9p21 in gastric adenocarcinoma. Results The overall incidence of MSI was 27% (64/233) in gastric cancer and 18% (42/233) in high grade intraepithelial neoplasia. MSI did not occur in normal tissues. The incidence of MSI in gastric cancer was significantly higher than that of high-grade intraepithelial neoplasia (P <0.05). In gastric cancer tissues, the incidence of MSI in 5 microsatellite loci was D9S171 (45%), IFNA (41%), D9S941 (22%), D9S166 (16%) and D9S942 Within the neoplastic group, IFNA (22%), D9S171 (21%), D9S941 (18%), D9S166 (14%) and D9S942 (11%) were followed. The incidence of MSI in D9S171 was 45% in gastric cancer and 21% in high-grade intraepithelial neoplasia. The difference was statistically significant (P <0.05). Conclusion The MSI of chromosome 9p21 in gastric cancer occurs at a high frequency. MSI may be an early molecular event in the development of gastric cancer and continues to play a role in the development of gastric cancer. p14, p15 and p16 genes may be related to the occurrence and development of gastric cancer.