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胎儿细胞存在于母体循环中和胎儿淋巴样细胞经胎盘迁移入母体是一个有争议的问题,尽管在人体和动物都有许多明确的观察报告。为检测母体循环中胎儿细胞,借助核型分类或用芥子喹吖因染色有丝分裂以鉴定母血中的男性XY细胞。本研究的目的是要找到一种培养方法,通过此法胎儿细胞可从母血中浓集,并用于产前诊断染色体异常和胎儿性别。对46名健康孕妇,在妊娠第7和第36周间检查其血中是否存在胎儿细胞。28人为初产妇,18人为经产妇。平均年龄为24±4.5岁。用于测定母体核型的淋巴细胞,由Autio和Schr(?)der常规微量培养技术处理。这种方法称为“常规培养”。
The presence of fetal cells in the maternal circulation and the migration of fetal lymphoid cells into the maternal via the placenta is a controversial issue, although there are many clear observations in humans and animals. To detect fetal cells in the maternal circulation, male XY cells in maternal blood are identified by karyotyping or by mitotic staining with mustard quinacridone. The purpose of this study was to find a culture method by which fetal cells can be concentrated from maternal blood and used for prenatal diagnosis of chromosomal abnormalities and fetal sex. Forty-six healthy pregnant women were examined for the presence of fetal cells in their blood between the 7th and 36th weeks of gestation. 28 were primipara and 18 were maternal. The average age was 24 ± 4.5 years. Lymphocytes used to determine maternal karyotype were processed by Autio and Schr (?) Der conventional micro-culture techniques. This method is called “routine culture.”