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以1月龄SD大鼠为实验对象,探讨了新鲜分离大鼠背根神经细胞的方法和影响因素。指出酶制剂的质量和剂量、处理的时间和温度、细胞灌流液的离子成份,是几个关键因素。
One-month-old SD rats were used as experimental subjects to explore the methods and influential factors of freshly isolated rat dorsal root neurons. Pointed out that the quality and dose of enzyme preparation, treatment time and temperature, ionic composition of cell perfusion fluid, is a key factor.